Cost¶
This page lists rough prices per unit of common consumables. Compare it to the time you spend doing experiments. Is it worth adding a few more controls and save your time in case something goes wrong? Or should you plan everything meticulously because you only have one chance to run your experiment?
| Category | Consumable | Unit | Cost per unit (€) | Source |
|---|---|---|---|---|
| Plasticware | Pipette tips | 1 tip | .1 | https://www.fishersci.de/shop/products/finntip-non-filtered-universal-pipette-tips/10549882 |
| 1.5 mL Eppendorf | 1 tube | .06 | https://www.fishersci.de/shop/products/eppendorf-snap-cap-microcentrifuge-biopur-safe-lock-tubes-3/15625367 | |
| PCR tube | 1 tube | .1 | https://www.fishersci.de/shop/products/0-2ml-pcr-tube-strips/p-3599559 | |
| Electroporation cuvette | 1 cuvette | 5.76 | https://www.fishersci.de/shop/products/fisherbrand-electroporation-cuvettes-plus-3/15532423 | |
| Gloves | 1 pair | .22 | https://www.fishersci.de/shop/products/indigo-nitrile-disposable-gloves-5/17919980 | |
| Petri dish | 1 dish | .25 | https://www.fishersci.de/shop/products/plastic-low-form-triple-vented-petri-dishes/12694785 | |
| Synthetic DNA | Oligos, 20 nt | 10 nmol | 2 | Genewiz |
| Plasmid | 1 plasmid | 150 | Genewiz | |
| Small reagents | Phusion Plus | .5 uL | 1.26 | https://www.fishersci.de/shop/products/phusion-plus-dna-polymerase-3/17218570 |
| dNTP | 1 uL | .11 | https://www.fishersci.de/shop/products/fermentas-dntp-set/p-4526908 | |
| T4 DNA Ligase | 1 uL | .31 | https://www.fishersci.de/shop/products/t4-dna-ligase/10621441 | |
| BsaI-HF-v2 (NEB) | 1 uL | 2.16 | https://www.fishersci.de/shop/products/x1000-units-bsai-hf-v2/15875754 | |
| BfuI (ThermoFisher) | 1 uL | 2.59 | https://www.fishersci.de/shop/products/bfui-bcivi/10784491 | |
| Dyes | TriTrack | 1 uL | .01 | https://www.fishersci.de/shop/products/fermentas-6x-tritrack-dna-loading-dye/11581575 |
| RNA Loading Dye (NEB) | 1 uL | .02 | https://www.fishersci.de/shop/products/4-ml-rna-loading-dye-2x-/17272574 | |
| SYBR Safe | 5 uL | .0002 | https://www.fishersci.de/shop/products/invitrogen-sybr-safe-dna-gel-stain-1/10328162 | |
| SYBR Gold | 5 uL | .0003 | https://www.fishersci.de/shop/products/invitrogen-sybr-gold-nucleic-acid-gel-stain-10-000x-concentrate-dmso/10358492 | |
| Bulk reagents | Agarose | 100 mg | .11 | https://www.fishersci.de/shop/products/fermentas-topvision-agarose/10588360 |
| 40% acrylamide | 1 mL | .20 | https://www.fishersci.de/shop/products/acrylamide-40-solutions/15484859 | |
| TEMED | 10 uL | .03 | https://www.fishersci.de/shop/products/pierce-tetramethylethylenediamine-temed/11836724 | |
| Kits | GeneJET PCR purification | 1 prep | 1.93 | https://www.fishersci.de/shop/products/fermentas-genejet-pcr-purification-kit/10400450 |
| Monarch plasmid purification | 1 prep | 2.30 | https://www.fishersci.de/shop/products/monarch-plasmid-miniprep-kit-50-preps-/15600557 | |
| NEBNext 96 IndexPrimers | 1 well | 11.59 | https://www.fishersci.de/shop/products/nebnext-multiplex-oligos-for-illumina-96-indexprimers-/16864421 | |
| NEBNext Ultra II DNA Prep Kit | 1 prep | 37.38 | https://www.fishersci.de/shop/products/nebnext-ultra-ii-dna-library-prep-kit-forillumina/15779914 | |
| AMPure XP Beads | 50 uL | 1.25 | https://www.fishersci.de/shop/products/agencourt-ampure-xp-3/p-7145017 | |
| Qubit HS kit | 1 uL | 1.27 | https://www.fishersci.de/shop/products/qubit-1x-dsdna-hs-br-assay-kits/15850210 | |
| P3 200-cycle NGS kit | 1 | =3229/44 |