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Solutions

Compound Amount (g) Milli-Q vol. (mL) MW (g/mol) Final conc. (M) Notes
NaOH 40 100 40 10 Add pellets slowly – the reaction is highly exothermic!
NaCl 5.87 100 58.7 1
CaCl2 11.08 100 110.8 1 Autoclave
MgSO4 12.03 100 120.3 1 Autoclave
Isopropyl β-D-1-thiogalactopyranoside (IPTG) 2.383 10 283.3 1 Filter-sterilize with .22 um